CBTF

ALDRICH/807664

Synonym: APN-TFS ester; Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate

Empirical Formula (Hill Notation): C₁₆H₄F₄NNaO₅S
Molecular Weight: 421.26
Linear Formula: C₁₆H₄F₄NNaO₅S
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-807664-50MG	50 mg	$228.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	powder
functional group	ester
InChI	1S/C16H5F4NO5S.Na/c17-10-12(19)15(27(23,24)25)13(20)11(18)14(10)26-16(22)9-5-3-8(4-6-9)2-1-7-21;/h3-6H,(H,23,24,25);/q;+1/p-1
InChI key	YFJYSJRZDOWXDH-UHFFFAOYSA-M
Quality Level	100
reaction suitability	reagent type: cross-linking reagent
SMILES string	O=C(OC1=C(F)C(F)=C(S(=O)(O[Na])=O)C(F)=C1F)C2=CC=C(C#CC#N)C=C2
storage temp.	−20°C

Application:	CBTF is a bifunctional crosslinker for amine-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. CBTF readily reacts with organic amines in basic conditions to produce APN-probes suitable for cysteine labeling.
General description:	CBTF (Sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate) is a heterobifunctional reagent. It bears a 3-arylpropionitrile (APN) group. CBTF can be prepared from 4-iodobenzoic acid. It participates in various amine-to-thiol coupling reactions. It participates in the synthesis of various antibody-dye conjugates. CBTF is widely used for antibody conjugation.
Other Notes:	Standard protein labeling procedure (lysine labeling): • Dissolve the protein in an appropriate buffer* with pH 7.5-9.0 (e.g. PBS) at 1-10mg/mL concentration. • Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per lysine residue). • Incubate at room temperature for 2 hours. • If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration. • The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours. Standard protein labeling procedure (cysteine labeling): Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10mg/mL concentration. Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue). Incubate at room temperature for 2 hours. If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration. *Note: avoid amine- and thiol-containing buffers.
Packaging:	50 mg in glass bottle

Symbol	GHS06
Signal word	Danger
Hazard statements	H301
Precautionary statements	P301 + P310 + P330
RIDADR	UN 2811 6.1 / PGIII
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352200

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