APN-COCI

ALDRICH/807745

Synonym: 4-(cyanoethynyl)benzoyl chloride

Empirical Formula (Hill Notation): C₁₀H₄ClNO
Molecular Weight: 189.60
Linear Formula: C₁₀H₄ClNO
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-807745-50MG	50 mg	$153.00 1/EAAdd To Favorites		Add To Favorites

form	powder
InChI	1S/C10H4ClNO/c11-10(13)9-5-3-8(4-6-9)2-1-7-12/h3-6H
InChI key	OWNJCJKKKZXTIX-UHFFFAOYSA-N
Quality Level	100
reaction suitability	reagent type: cross-linking reagent
SMILES string	N#CC#CC1=CC=C(C(Cl)=O)C=C1
storage temp.	2-8°C

Application:	APN-COCl is a bifunctional reagent appropriate for the introduction of APN function.
Other Notes:	The coupling between an amine and APN-COCl should be performed in dry organic solvents (e.g. THF) in the presence of a base (e.g. TEA). The resulting conjugate can be readily coupled with thiol-containing biomolecules using the following protocol: • Dissolve the protein in the appropriate buffer with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration. • Apply the appropriate amount of the stock solution of the APN-labeled molecule (1-5 molar eq. per free cysteine residue). • Incubate at room temperature for 2 hours. If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.
Packaging:	50 mg in glass bottle

Symbol	GHS05,GHS07
Signal word	Danger
Hazard statements	H302 - H314
Precautionary statements	P260 - P280 - P301 + P312 + P330 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338
RIDADR	UN 3261 8 / PGII
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable