5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

ALDRICH/809705

Synonym: 5-DBCO-PEG₄-dUTP

Empirical Formula (Hill Notation): C₄₂H₅₄N₅O₂₁P₃
Molecular Weight: 1057.82
Linear Formula: C₄₂H₅₄N₅O₂₁P₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-809705-50UL	50 µL	$414.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	>95% (HPLC)
form	solution
Quality Level	200
reaction suitability	reaction type: click chemistry
shipped in	wet ice
SMILES string	O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=CCNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O
solubility	Tris buffer: 100 mM (pH 7.5)
storage temp.	−20°C

Application:

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

Packaging:

50 μL in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Purity	>95% (HPLC)
Storage Temp.	−20°C
UNSPSC	12352200

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