Azure A chloride
ALDRICH/861049 - certified by the Biological Stain Commission, Dye content 70 %
Synonym: Methylene blue
CAS Number: 531-53-3
Empirical Formula (Hill Notation): C14H14ClN3S
Molecular Weight: 291.80
EC Number: 208-510-7
MDL Number: MFCD00012112
Linear Formula: C14H14ClN3S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861049-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861049-5G
| ε (extinction coefficient) | ≥11000 at 242-246 nm in H2O at -1 |
| ≥26000 at 287-291 nm in H2O | |
| ≥34000 at 620-634 μm in H2O | |
| λmax | 633 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | blue to very dark blue |
| very dark green | |
| composition | Dye content, 70% |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C14H13N3S.ClH/c1-17(2) |
| InChI key | NALREUIWICQLPS-UHFFFAOYSA |
| mp | 290 °C (dec.) (lit.) |
| Quality Level | 200  |
| SMILES string | NC1=CC(SC(C2=N3)=CC(C=C2) |
| solubility | H2O: 1 mg/mL |
| storage temp. | room temp |
| Application: | Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles. |
| Biochem/physiol Actions: | The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye. |
| Packaging: | 1, 5, 25 g in glass bottle |
| Suitability: | Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 290 °C (dec.) (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 52005 |
| UNSPSC | 12171500 |