3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate
ALDRICH/861510 - 97%
Synonym: TMB dihydrochloride
CAS Number: 207738-08-7
Empirical Formula (Hill Notation): C16H20N2 · 2HCl · xH2O
Molecular Weight: 313.27 (anhydrous basis)
MDL Number: MFCD00150104
Linear Formula: [-C6H2(CH3)2-4-NH2]2·2HCl·xH2O
Product Type: Chemical
Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 861510-1G
| assay | 97% |
| form | powder |
| InChI | 1S/C16H20N2.2ClH.H2O/c1-9 |
| InChI key | KVCWTKDFVVSVSJ-UHFFFAOYSA |
| SMILES string | O.Cl.Cl.Cc1cc(cc(C)c1N)-c |
| storage temp. | 2-8°C |
| Application: | Convenient water-soluble form of TMB for determination of peroxidase. |
| Application: | Noncarcinogenic substitute for benzidine. |
| Packaging: | 1 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 97% |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352100 |