2-(Azidomethyl)nicotinic acid imidazolide
ALDRICH/913812 - ≥95%
Synonym: 2-(Azidomethyl)-3-(1H-
CAS Number: 1612756-29-2
Empirical Formula (Hill Notation): C10H8N6O
Molecular Weight: 228.21
MDL Number: MFCD31560475
Linear Formula: C10H8N6O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 913812-50MG.
| assay | ≥95% |
| form | powder |
| mp | 74-80 °C |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | 2-(Azidomethyl)nicotinic acid imidazolide (NAI-N3) is an RNA icSHAPE probe for live-cell RNA structure profiling across the genome. icSHAPE -- or in vivo click selective 2′-hydroxyl acylation and profiling experiment -- uses NAI-N3 in a chemoaffinity method to probe RNA structure. NAI-N3 is an azido version of the cell-permeable SHAPE reagent 2-methylnicotinic acid imidazolide (NAI) that permits the tagging of NAI-N3-modified RNA with a biotin moiety for subsequent capture via streptavidin. Not only do such strategies further the understanding of RNA structure in living cells but also provide a tool for identifying regions that may be susceptible to therapeutic targeting. Recently, the azidomethylnicotinyl (AMN) group of NAI-N3 was demonstrated to block the function of gRNA and CRISPR systems, which could be reactivated by removing the AMN groups with Staudinger reduction (DPBM phosphine), overall providing a means to control nucleic acid cleavage and gene editing in live cells. |
| Other Notes: | Conditional control of RNA-guided nucleic acid cleavage and gene editing  SnapShot: RNA Structure Probing Technologies Multiplex Aptamer Discovery through Apta-Seq and Its Application to ATP Aptamers Derived from Human-Genomic SELEX Structural imprints in vivo decode RNA regulatory mechanisms |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H242 - H315 - H319 |
| Precautionary statements | P210 - P235 - P302 + P352 - P305 + P351 + P338 - P370 + P378 - P403 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% |
| mp | 74-80 °C |
| Storage Temp. | −20°C |