Fluorescein (free acid)
ALDRICH/F2456 - Dye content 95 %
CAS Number: 2321-07-5
Empirical Formula (Hill Notation): C20H12O5
Molecular Weight: 332.31
EC Number: 219-031-8
MDL Number: MFCD00005050
Linear Formula: C20H12O5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F2456-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F2456-2.5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: F2456-100G
| ε (extinction coefficient) | ≥12000 at 282-286 nm |
| ≥39000 at 236-240 nm | |
| ≥70000 at 488-492 nm | |
| λmax | 496 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | orange to red-brown |
| composition | Dye content, 95% |
| form | powder |
| InChI | 1S/C20H12O5/c21-11-5-7-15 |
| InChI key | GNBHRKFJIUUOQI-UHFFFAOYSA |
| mp | 320 °C (lit.) |
| Quality Level | 200  |
| SMILES string | Oc1ccc2c(Oc3cc(O)ccc3C24O |
| storage temp. | room temp |
| technique(s) | titration: suitable |
| Application: | Fluorescein (free acid) has been used as a staining agent in retinal imaging. |
| Application: | Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard. |
| General description: | Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety. |
| Packaging: | 2.5, 100, 500 g in glass bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 320 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 45350:1 |
| UNSPSC | 12171500 |