DCA Deblock
ALDRICH/L021000 - with Dichloromethane
Product Type: Chemical
| color | clear |
| concentration | 42.2-51.6 g/L (DCA content, titration) |
| form | liquid |
| impurities | ≤200 ppm water content (Karl Fischer) |
| product line | Proligo Reagents |
| storage condition | dry at room temperature |
| type | for DNA synthesis |
| General description: | Deblock solution contains dichloroacetic acid (DCA) in dichloromethane that assists in the removal of the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, before the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction. |
| Other Notes: | Deblock solution containing dichloroacetic acid (DCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction. |
| Preparation Note: | Dicloromethane / Dichloroacetic acid 97/3 (v/v) |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H315 - H318 - H336 - H351 - H360FD - H400 |
| Precautionary statements | P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | C,N |
| Risk Statements | 34-37-40-50-67 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN2922 - class 8 - PG 3 - EHS - acidic - Corrosive liquids, toxi |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41116004 |