Cap A
ALDRICH/L040050 - configured for (ÄKTA® and OligoPilot®)
Product Type: Chemical
| color | clear |
| compatibility | configured for (ÄKTA® and OligoPilot®) |
| impurities | ≤100 ppm water content (Karl Fischer) |
| product line | Proligo Reagents |
| Quality Level | 200  |
| storage condition | dry at room temperature |
| suitability | conforms to structure (GC, Formulation) |
| type | for DNA synthesis |
| Application: | Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps. |
| General description: | Cap A solution contains acetic anhydride and tetrahydrofuran (THF). |
| Legal Information: | ÄKTA is a registered trademark of Cytiva |
| Legal Information: | OligoPilot is a registered trademark of Cytiva |
| Other Notes: | Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst. |
| Preparation Note: | Acetonitrile / N-Methylimidazole 8/2 (v/v) |
| Symbol |    GHS02,GHS05,GHS06 |
| Signal word | Danger |
| Hazard statements | H225 - H302 + H332 - H311 - H314 |
| Precautionary statements | P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| RIDADR | UN 2924 8(3) / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 35.6 °F |
| Flash Point(C) | 2 °C |
| UNSPSC | 12352200 |