NHS-dPEG®4-biotin
ALDRICH/QBD10200
Synonym: (+)
CAS Number: 459426-22-3
Empirical Formula (Hill Notation): C25H40N4O10S
Molecular Weight: 588.67
Linear Formula: C25H40N4O10S
Product Type: Chemical
| assay | >90% |
| form | solid or viscous liquid |
| InChI | 1S/C25H40N4O10S/c30-20(4- |
| InChI key | DTLVBHCSSNJCMJ-JXQFQVJHSA |
| polymer architecture | shape : linear functionality : monofunctional |
| reaction suitability | reagent type: cross-linking reagent reaction type: Biotinylations reactivity: amine reactive |
| reagent type: linker | |
| shipped in | ambient |
| SMILES string | O=C(ON1C(CCC1=O)=O)CCOCCO |
| storage temp. | −20°C |
| Features and Benefits: | NHS-dPEG®4-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG®4-biotin has a long spacer of precise length (19.2 Å) between the biotin moiety and the labeled molecule. This dPEG® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin. |
| General description: | NHS-dPEG®4-biotin is a water-soluble cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®4-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies. |
| Legal Information: | dPEG is a registered trademark of Quanta BioDesign |
| Legal Information: | Products Protected under U.S. Patents # 7,888,536 B2 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% |
| Storage Temp. | −20°C |
| UNSPSC | 12161502 |