DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation
ROCHE/03353575910 - sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| Quality Level | 100 |
| shipped in | dry ice |
| sustainability | Greener Alternative Product |
| usage | sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide) |
| Application: | DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation is for 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant terminal transferase. DIG-labeled oligonucleotides has been used in a variety of hybridization techniques: • dot/slot blots • colony/ plaque hybridizations • Southern blots/ northern blots • in situ hybridizations |
| Features and Benefits: | • Fast hybridization kinetics, due to the small size of oligonucleotides • Single-stranded probes, no renaturation during hybridization • Sequence can be designed according to the experiment • Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells |
| General description: | The DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates (ddUTP) to the 3′-OH end of oligonucleotides. Thus, helps in labeling oligonucleotides. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 9 components |
| Preparation Note: | Activator: sodium sulfate, Tris Working concentration: Oligonucleotides: 100 pmol Up to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction. |
| Preparation Note: | Assay Time: The complete procedure from labeling the oligonucleotide to hybridization and detection of the first visible signal can be accomplished within less than 24 hours. Sample Materials Oligonucleotides of a length from 14 to 100 nucleotides, purified by HPLC or gel electrophoresis |
| Principle: | One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction. |
| Quality: | Function tested in a dot blot. |
| Storage and Stability: | Store at -15–-25 °C. (unopened kit) |
| Symbol |    GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 + H332 - H350i - H360F - H411 |
| Precautionary statements | P201 - P261 - P273 - P280 - P308 + P313 - P391 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 12352200 |
| Components | Reaction Buffer 5x concentrated; CoCl<sub>2</sub> Solution 25 mM; DIG-ddUTP Solution 1 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl; Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA |