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DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation

ROCHE/03353575910 - sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-3353575910 25 reactions
$738.00
1/EA
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manufacturer/tradename Roche
Quality Level 100 
shipped in dry ice
usage sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)
Application: DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation is for 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant terminal transferase.
DIG-labeled oligonucleotides has been used in a variety of hybridization techniques:
• dot/slot blots
• colony/ plaque hybridizations
• Southern blots/ northern blots
• in situ hybridizations
Features and Benefits: • Fast hybridization kinetics, due to the small size of oligonucleotides
• Single-stranded probes, no renaturation during hybridization
• Sequence can be designed according to the experiment
• Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells
General description: The DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates (ddUTP) to the 3′-OH end of oligonucleotides. Thus, helps in labeling oligonucleotides.
General description: We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: 1 kit containing 9 components
Preparation Note: Activator: sodium sulfate, Tris
Working concentration: Oligonucleotides: 100 pmol
Up to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction.
Preparation Note: Assay Time: The complete procedure from labeling the oligonucleotide to hybridization and detection of the first visible signal can be accomplished within less than 24 hours.

Sample Materials
Oligonucleotides of a length from 14 to 100 nucleotides, purified by HPLC or gel electrophoresis
Principle: One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.
Quality: Function tested in a dot blot.
Storage and Stability: Store at -15–-25 °C. (unopened kit)
UNSPSC 12352200
Components Reaction Buffer 5x concentrated; CoCl<sub>2</sub> Solution 25 mM; DIG-ddUTP Solution 1 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl; Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA

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