DIG Oligonucleotide Tailing Kit, 2nd generation
ROCHE/03353583910 - sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Product Type: Chemical
greener alternative category | Aligned ![]() ![]() |
greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry ![]() |
manufacturer/tradename | Roche |
Quality Level | 100 ![]() |
storage condition | avoid repeated freeze/thaw cycles |
usage | sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide) |
Application: | DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in: • northern blot assay • in situ hybridization (ISH) • fluorescence in situ hybridization (FISH) In addition to common hybridization techniques, DIG-labeled oligonucleotides are especially useful for screening expression libraries for sequence-specific DNA binding proteins, for example, transcription factors. |
Features and Benefits: | Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100). • Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides • Fast hybridization kinetics, due to the small size of oligonucleotides • Single-stranded probes, no renaturation during hybridization • Sequence can be designed according to the experiment • Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells |
General description: | DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides. |
General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Packaging: | 1 kit containing 11 components |
Preparation Note: | Working concentration: Oligonucleotides In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied. |
Principle: | DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s). |
Storage and Stability: | Store at -15–-25 °C. (unopened kit) |
Symbol | ![]() ![]() ![]() |
Signal word | Danger |
Hazard statements | H302 + H332 - H350i - H360F - H411 |
Precautionary statements | P201 - P261 - P273 - P280 - P308 + P313 - P391 |
RIDADR | UN 3316 9 |
WGK Germany | WGK 3 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
UNSPSC | 12352200 |
Components | Reaction Buffer 5x concentrated; CoCl<SUB>2</SUB> Solution 25 mM; DIG-dUTP Solution 1 mM; dATP Solution 10 mM; Recombinant Terminal Transferase 400 U/μl; Control Oligonucleotide, unlabeled 20 pmol/μl; Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl; Control DNA 0.25 mg/ml; Glycogen Solution 20 mg/ml; DNA Dilution Buffer, 50 μg/ml fish sperm DNA; Poly(A) Solution 10 mg/ml |