Streptavidin Mutein Matrix
ROCHE/03708152001 - suspension, pkg of 5 mL
Product Type: Chemical
| form | suspension |
| manufacturer/tradename | Roche |
| packaging | pkg of 5 mL |
| parameter | 4-25 °C optimum reaction temp. |
| shipped in | wet ice |
| Application: | • Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteins • Has been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation Kits • Is convenient for purification of chemically biotinylated proteins • It was also used in single-step affinity chromatography and affinity chromatography. |
| Features and Benefits: | • Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions. • Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers. • Economic: High binding capacity and multiple reuse allows economic handling of the matrix. |
| General description: | Contents Settled resin of 50% suspension Streptavidin Mutein Matrix. |
| General description: | Streptavidin mutein, immobilized. |
| General description: | The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(Kd 0.6 x 10-15 M (avidin), 4 x 10-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidi Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10-7 M. The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Storage and Stability: | Store at 2 to 8 °C. (Do not freeze!) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 12352200 |