Dideoxynucleoside Triphosphate Set
ROCHE/03732738001 - Sequencing Grade, sodium salt
Product Type: Chemical
| assay | ≤1.5% (ddNDP, HPLC) |
| ≥98% (ddNTP, HPLC) | |
| color | colorless |
| form | liquid |
| grade | Molecular Biology |
| manufacturer/tradename | Roche |
| packaging | pkg of 4 × 100 μL (4 x 1 μmol; 10 mM, each) |
| shipped in | dry ice |
| solubility | water: miscible |
| storage temp. | −20°C (−15°C to −25°C) |
| Analysis Note: | Each lot of dideoxynucleotide solution is assayed for function in sequencing reaction. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures. |
| Application: | 2′,3′-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase (e.g., Klenow enzyme) and are therefore used for DNA sequencing according to Sanger (Sanger, F. et al. (1977) PNAS USA 74, 5463). Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four reactions are set up, each containing the same template and primer, and a chain terminator specific for A, C, G, or T. Because only a small amount of the chain terminator is included, incorporation into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, and every DNA strand will end at the same type of base (A, C, G, or T). |
| General description: | The Dideoxynucleoside Triphosphate Set, Sequencing Grade, sodium salt solution, consists of dideoxynucleotides of very high purity (ddNTP HPLC, area% ≥98%; ddNDP HPLC, area% ≤1.5%), specifically manufactured and tested for sequencing reactions. The set contains 4 individual vials of ddATP, ddCTP, ddGTP, and ddTTP, each at 10 mM as a clear, colorless solution of the sodium salt (pH 8.3). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | |
| Preparation Note: | Activator: Mg2+, Co2+ and Mn2+ ions Working solution: Preparation of Termination Mix Before preparing the termination mix, dilute an appropriate volume of each dideoxynucleotide in water to a concentration of 10 μM. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≤1.5% (ddNDP, HPLC); ≥98% (ddNTP, HPLC) |
| Storage Temp. | −20°C (−15°C to −25°C) |
| UNSPSC | 12352204 |
| Components | ddATP, sodium salt 10 mM; ddCTP, sodium salt 10 mM; ddGTP, sodium salt 10 mM; ddTTP, sodium salt 10 mM |