Adenosine Deaminase (ADA)
ROCHE/10102105001 - from calf intestine
Synonym: ADA
Product Type: Chemical
biological source | bovine (calf) intestine |
form | solution |
manufacturer/tradename | Roche |
mol wt | 230-440 kDa (high) |
30-47 kDa (low ) | |
optimum pH | 7.0-7.4 |
packaging | pkg of 2 mL (10 mg) |
Quality Level | 100 ![]() |
shipped in | wet ice |
specific activity | ~200 units/mg protein (At 25 °C with adenosine as the substrate.) |
storage temp. | 2-8°C |
Application: | Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages. |
Application: | Adenosine deaminase has been used to pre-treat primary Kupffer cells. |
Biochem/physiol Actions: | Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia. |
Biochem/physiol Actions: | Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine. |
General description: | Adenosine deaminase (ADA) is a zinc metalloenzyme. |
General description: | Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Physical form: | Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6 |
Preparation Note: | Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C. |
Quality: | Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
activity | specific activity: ~200 units/mg protein (At 25 °C with adenosine as the substrate.) |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 3.5.4.4 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |