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DNase I

ROCHE/10104159001 - grade II, from bovine pancreas

Synonym: Deoxyribonuclease; Deoxyribonucleate 5′-oligonucleotido-hydrolase

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10104159001 100 mg
$95.10
1/EA
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DNase I

 

biological source bovine pancreas
form lyophilized
manufacturer/tradename Roche
optimum pH ~7.0
packaging pkg of 100 mg
Quality Level 100 
storage temp. 2-8°C
technique(s) DNA extraction: suitable
Application: Isolation procedures for proteins (e.g., membrane proteins).
Bovine pancreatic deoxyribonuclease I (DNase I) has been used for-
• The isolation of cells from lung, skin and tumor samples
• Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †
General description: Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Activator: Ca2+ (0.12 mM) in combination with Mg2+ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)].
Working concentration: 1 mg/ml
Do not vortex while dissolving!
Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Unit Definition: One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.
Storage Temp. 2-8°C
UNSPSC 12352204

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