DNase I

ROCHE/10104159001 - grade II, from bovine pancreas

Synonym: Deoxyribonuclease; Deoxyribonucleate 5′-oligonucleotido-hydrolase

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-10104159001	100 mg	$98.60 1/EAAdd To Favorites		Add To Favorites

DNase I

biological source	bovine pancreas
form	lyophilized
manufacturer/tradename	Roche
optimum pH	~7.0
packaging	pkg of 100 mg
Quality Level	100
storage temp.	2-8°C
technique(s)	DNA extraction: suitable

Application:	Isolation procedures for proteins (e.g., membrane proteins). Bovine pancreatic deoxyribonuclease I (DNase I) has been used for- • The isolation of cells from lung, skin and tumor samples • Bacterial DNA extraction from live bacterial cells that are resistant to DNase I †
General description:	Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin. It is a double-strand-specific endonuclease that requires bivalent cations for maximal activity.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Activator: Ca²⁺ (0.12 mM) in combination with Mg²⁺ enhances activity [Laskowski (1971); Melgar and Goldthwait (1968)]. Working concentration: 1 mg/ml Do not vortex while dissolving! Storage conditions (working solution): Reconstituted in water, the solution can be kept for 2 to 3 days at 2 to 8 °C. Do not vortex during dissolving. Note: Dissolve at least 1 mg/ml.
Unit Definition:	One unit is the enzyme activity that causes an increase in the absorbance of 0.001 per minute under assay conditions.