β-Galactosidase
ROCHE/10105031001 - from E. coli overproducer
Synonym: β-galactosidase; β-galactosidase
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | Escherichia coli |
| concentration | 5.00 mg/mL |
| form | suspension |
| manufacturer/tradename | Roche |
| mol wt | 540 kDa |
| optimum pH | 7 |
| packaging | pkg of 1,500 U |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| suitability | suitable for UV spectrophotometry and general use |
| technique(s) | activity assay: suitable |
| Application: | β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays. |
| General description: | At 37 °C with 2-nitrophenyl-β-D-galacto |
| General description: | β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline |
| Preparation Note: | The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose. In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges. Activator: K (50 mM) is required for activation (lactose hydrolysis). Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galacto |
| Quality: | Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each |
| Specificity: | Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.23 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |