GTP
ROCHE/10106399001 - >90% (HPLC), crystals, pkg of 250 mg
Synonym: Guanosine 5′-triphosphate sodium salt hydrate; 5′-GTP-Na2
CAS Number: 36051-31-7
Empirical Formula (Hill Notation): C10H16N5O14P3 · xNa+ · yH2O
Molecular Weight: 523.18 (anhydrous free acid basis)
MDL Number: MFCD00149227
Linear Formula: C10H16N5O14P3 · xNa+ · yH2O
Product Type: Chemical
| assay | >90% (HPLC) |
| description | Disodium salt |
| form | crystals |
| InChI | 1S/C10H16N5O14P3.2Na.2H2O |
| InChI key | HPLNHYUZFVUXFJ-ZVQJTLEUSA |
| manufacturer/tradename | Roche |
| mol wt | Mr 523.2 (GTP) |
| Mr 567.1 (GTP-Na2) | |
| packaging | pkg of 250 mg |
| Quality Level | 100  |
| SMILES string | O.O.[Na+].[Na+].NC1=Nc2c( |
| storage condition | avoid repeated freeze/thaw cycles |
| Application: | GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents. |
| General description: | GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly. |
| General description: | GTP is supplied as the disodium salt in crystal form. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction. Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3. If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen. Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference. |
| Quality: | Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >90% (HPLC) |
| UNSPSC | 12352200 |