NADH
ROCHE/10107735001 - 100% (dry weight), amorphous powder, pkg of 500 mg
Synonym: β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate; β-DPNH; β-NADH; DPNH; Diphosphopyridine nucleotide, reduced form; NADH
CAS Number: 606-68-8 (anhydrous)
Empirical Formula (Hill Notation): C21H27N7Na2O14P2 · xH2O
Molecular Weight: 709.40 (anhydrous basis)
MDL Number: MFCD03791273
Linear Formula: C21H27N7Na2O14P2 · xH2O
Product Type: Chemical
λmax | 260 and 340 nm |
assay | 100% (dry weight) |
description | Grade I, disodium salt |
form | amorphous powder |
InChI | 1S/C21H29N7O14P2.2Na.H2O/ |
InChI key | FWBNCIFELNNJCX-UHOVGGJYSA |
manufacturer/tradename | Roche |
mol wt | Mr 665.4 (NADH) |
Mr 709.4 (NADH-Na2) | |
packaging | pkg of 500 mg |
pH | 7.5 ± 0.5 (100 mg/mL in water) |
7.5 (100 mg/mL in water, ±0.5) | |
Quality Level | 100 |
shipped in | wet ice |
SMILES string | O.[Na+].[Na+].NC(=O)C1=CN |
storage temp. | 2-8°C |
Analysis Note: | Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at Hg 334 nm, 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at Hg 365 nm |
Application: | As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
Application: | Nicotinamide adenine dinucleotide (NADH) has been used in: • nicotinamide adenine dinucleotide (NAD+) /NADH cycling assay. • adenosine triphosphate (ATP)ase assay. • pyruvate kinase enzyme assay. |
Biochem/physiol Actions: | NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
Other Notes: | Shipping conditions may vary |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Packaging: | Packaged by solid weight. |
Preparation Note: | Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C. |
Quality: | Contaminants: Relative reaction with LDH: 1.00 ± 0.05 (referred to a standard) Organic solvents: <4% (GC) |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | 100% (dry weight) |
Storage Temp. | 2-8°C |
UNSPSC | 12352204 |