Nuclease S7
ROCHE/10107921001 - Micrococcal nuclease, from Staphylococcus aureus
Synonym: s7 nuclease
Product Type: Chemical
| biological source | Staphylococcus aureus |
| form | lyophilized |
| manufacturer/tradename | Roche |
| optimum pH | 7.5 |
| packaging | pkg of 15,000 U |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~15,000 U/mg |
| Application: | • Removal of endogenous RNA from in vitro translation systems • RNA sequencing experiments |
| Application: | Nuclease S7 has been used in ribonuclease selection process for ribosome profiling. |
| Biochem/physiol Actions: | Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Storage and Stability: | Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C. |
| Unit Definition: | One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~15,000 U/mg |
| UNSPSC | 12352204 |