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Nuclease S7

ROCHE/10107921001 - Micrococcal nuclease, from Staphylococcus aureus

Synonym: s7 nuclease

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-10107921001 15000 units
 $259.00
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biological source Staphylococcus aureus
form lyophilized
manufacturer/tradename Roche
optimum pH 7.5
packaging pkg of 15,000 U
Quality Level 100 
shipped in wet ice
specific activity ~15,000 U/mg
Application: • Removal of endogenous RNA from in vitro translation systems
• RNA sequencing experiments
Application: Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.
Biochem/physiol Actions: Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Other Notes: One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.
Preparation Note: Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.
activity specific activity: ~15,000 U/mg
UNSPSC 12352204

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