Papain
ROCHE/10108014001 - from Carica papaya
Synonym: cystein protease; papain
Product Type: Chemical
| biological source | papaya |
| concentration | 10-100 mg/mL |
| form | suspension |
| manufacturer/tradename | Roche |
| mol wt | 23 kDa |
| optimum pH | 6.0-7.0 |
| packaging | pkg of 10 mL (100 mg) |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | 30 U/mg |
| ~30 units/mg protein (At 25 °C with BAEE as the substrate.) |
| Application: | Papain has been used in the digestion of various brain cells. |
| Application: | Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity. |
| Biochem/physiol Actions: | Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate. |
| Features and Benefits: | Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals |
| General description: | Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension, crystalline, nonsterile |
| Preparation Note: | Working concentration: 0.05 to 0.5 mg/ml |
| Storage and Stability: | Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months) |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 - P342 + P311 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 30 U/mg; specific activity: ~30 units/mg protein (At 25 °C with BAEE as the substrate.) |
| Enzyme Commission (EC) Number | 3.4.22.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |