Peroxidase (POD)
ROCHE/10108090001 - grade I, from horseradish
Synonym: POD; peroxidase
Product Type: Chemical
| biological source | horseradish |
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| optimum pH | 6.0-6.5 |
| packaging | pkg of 25,000 U |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.) |
| 250 U/mg | |
| storage temp. | 2-8°C |
| Application: | Oxidation of electron donors (e.g., epoxidation of double bonds). Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.It has been used in ligand blotting. |
| Application: | Peroxidase (POD) has been used in AUR (Amplex UltraRed assay) to measure extracellular H2O2 produced from disaggregated slug cells. |
| Biochem/physiol Actions: | Peroxidase (POD) is involved in the dehydrogenation of a large number of organic compounds including, phenols and aromatic amines, hydroquinones and hydroquinoid amines, mainly benzidine derivatives. |
| General description: | Donor:hydrogen-peroxide oxidoreductase |
| General description: | Peroxidase (POD) (E.C. 1.11.1.7) is extensively expressed in most fruits and vegetables. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents. Working solution: PBS (phosphate buffered saline) or water (starting concentration 20 mg/ml). |
| Quality: | Contaminants: <0.001% ATPase and acid phosphatase each, <0.7% catalase Purity number: approximately 3.0 (A403/A275) |
| Reconstitution: | Solution (10 mg/ml in water): clear, red brown; pH-value: 6.0 to 7.0. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥250 units/mg protein (At 25 °C with guaiacol and H2O2 as the substrates.); specific activity: 250 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |