Poly(A)
ROCHE/10108626001 - lyophilized, suitable for PCR, pkg of 100 mg
Synonym: Poly(A); Polyadenylic acid
Product Type: Chemical
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| absorbance ratio | A250/260 nm 0.86-0.90 |
| A280/260 nm 0.28-0.32 | |
| A290/260 nm 0.03-0.05 | |
| color | white |
| concentration | 0.5 mg/mL (Working concentration) |
| description | Polyadenylic acid |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 700-3500 kDa |
| packaging | pkg of 100 mg |
| Quality Level | 100  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| technique(s) | PCR: suitable |
| Application: | Poly(A) has been used for droplet digital PCR (ddPCR) assay. |
| Application: | Polyadenylic acid (Poly(A)) to inhibit Exo1′s exonuclease activity. It has also been used as a carrier to resuspend lyophilized gBlocks. |
| Biochem/physiol Actions: | Polyadenylic acid (Poly(A)) tails present at 3′ end are produced in the cell nucleus. It contains ~250 nucleotides in mammalian cells. Poly(A) regulates mRNA decay and the initiation of translation. Cytoplasmic poly(A) extension modulates translation. |
| General description: | Poly(A) is used as a carrier for quantitative precipitation of DNA and RNA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate, potassium salt |
| Preparation Note: | Working concentration: 0.5 mg/ml A concentration of 0.5 mg/ml is recommended. Working solution: 0.5 mg/ml is recommended. |
| Quality: | Typical analysis: 2.3μmol/mg Poly(A) (from absorbance) in relation to one mononucleotide unit. Chromatographically homogeneous. Absorbance determination A250/A260, A280/A260, A290/A260 |
| Sequence: | Chain Length 2.100 to 10.000 nucleotides |
| Unit Definition: | 1 A260 unit corresponds to 40 μg ssRNA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105600 |