RNase
ROCHE/10109134001 - from bovine pancreas
Synonym: Rnase
Product Type: Chemical
| biological source | bovine pancreas |
| form | dry powder (prepared from crystallized ribonuclease) |
| manufacturer/tradename | Roche |
| packaging | pkg of 500 mg |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).) |
| storage temp. | 2-8°C |
| Application: | RNase has been used in analysis of DNA fragmentation using agarose gel electrophoresis. It has been used during flow cytometry analysis. |
| Application: | RNase has been used in DNA ladder assay. |
| Biochem/physiol Actions: | Heat inactivation: 1 mM EDTA |
| General description: | Crude mixture of RNases.Dry powder (prepared from crystallized ribonuclease). |
| General description: | Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under the assay conditions; A0 to A1 corresponds to the total conversion, A1 being the final absorbance. |
| Preparation Note: | Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity. Working solution: It is recommended to dissolve the RNase (1 mg/ml) in water or TE buffer, pH 7.5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~40 U/mg dry powder (dry powder according to Kunitz (+25°C; RNA as substrate).) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |