Sorbitol Dehydrogenase (SDH)
ROCHE/10109339001 - from sheep liver
Synonym: SDH; Sorbitol Dehydrogenase; sorbitol
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | sheep liver |
| color | white |
| concentration | 0.16 mg/mL Biuret |
| foreign activity | ADH <0.00182% |
| GIDH <0.00152% | |
| Glucose-DH <0.00182% (NAD as coenz.) | |
| LDH <0.00167% | |
| MDH <0.00198% | |
| NADH oxidase 0.00532% | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 115 kDa |
| NCBI accession no. | XP_004010700   |
| optimum pH | 7.5 |
| packaging | pkg of 10 mg (60 mg lyophilizate) |
| Quality Level | 100 |
| solubility | water: 60 mg/mL, clear, colorless |
| specific activity | ~40 units/mg protein (At 25 °C with D-fructose as the substrate.) |
| storage temp. | 2-8°C |
| suitability | suitable for ELISA applications |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P07846 |
| Application: | Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses). |
| Biochem/physiol Actions: | Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of |
| General description: | L-iditol:NAD+ 5′-oxidoreductase Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose). |
| Preparation Note: | Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer. Stabilizers: Maltose is used as stabilizer. Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks. |
| Quality: | Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each. |
| Storage and Stability: | Store at 2 to 8 °C. (Store the lyophilizate dry.) |
| Unit Definition: | One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~40 units/mg protein (At 25 °C with D-fructose as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.14 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |