Trypsin Inhibitor
ROCHE/10109878001 - from chicken egg white
Synonym: trypisin
Product Type: Chemical
| biological source | chicken egg white |
| concentration | 1 mg/mL |
| form | powder |
| manufacturer/tradename | Roche |
| optimum pH | 3.8-4.5 |
| packaging | pkg of 1 g |
| Quality Level | 100  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures. |
| Application: | Trypsin Inhibitor has been used: • as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting • to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture • to neutralize trypsin post dissociation and trypsinization of brain tissues • termination of tissue disaggregation and for subcultivation procedures |
| General description: | Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 0 Working solution: Solvent is recommended in distilled water. Stock solution: 1 mg/ml Storage conditions (working solution): -15 to -25 °C Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles. |
| Reconstitution: | Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months. |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |