Trypsin Inhibitor
ROCHE/10109886001 - from soybean
Synonym: trypsin
Product Type: Chemical
| biological source | soybean |
| concentration | 1 mg/mL |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | Mr 20,100 |
| packaging | pkg of 50 mg |
| pH | 3.8-4.5 |
| Quality Level | 100  |
| solubility | water: soluble |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures. |
| Application: | Trypsin Inhibitor has been used: • in the buffer for hepatocyte isolation • to inhibit trypsin in Corneal epithelial cells • to inhibit papain activity while treating hippocampal neural stem cells (NSCs) |
| Biochem/physiol Actions: | Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin. |
| Biochem/physiol Actions: | Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds. |
| General description: | Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 10 to 100 μg/ml Working solution: Recommended solvent is distilled water. Stock solution: 1 mg/ml Storage conditions (working solution): -15 to -25 °C The enzyme is stable for 6 months, frozen in aliquots. |
| Reconstitution: | Sensitive to heat, alkaline pH, and protein-precipitating compounds. |
| Specifications: | Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate) Isoelectric point: 4.5 |
| Specificity: | Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases). |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| Unit Definition: | 1 U = 270 S & T-units or 90 USP-units |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |