Xanthine Oxidase (XOD)
ROCHE/10110434001 - from cow milk
Synonym: XOD
Product Type: Chemical
| biological source | bovine milk |
| form | suspension |
| manufacturer/tradename | Roche |
| optimum pH | 8.5-9.0 |
| packaging | pkg of 1 mL (20 U) |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~1 units/mg protein (At 25 °C with xanthine as the substrate.) |
| storage temp. | 2-8°C |
| Application: | Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR. |
| Application: | Xanthine Oxidase has been used to study tyrosine nitration. |
| Biochem/physiol Actions: | Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium. |
| General description: | Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8. |
| General description: | Xanthine:oxygen oxidoreductase |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8 |
| Preparation Note: | Activator: O2 Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added. |
| Quality: | Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate) Note: Chromatographically purified. |
| Sequence: | XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD. |
| Unit Definition: | One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E293nm = 12.2 mmol -1 x L x cm-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~1 units/mg protein (At 25 °C with xanthine as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.3.22 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |