Glucose-6-Phosphate Dehydrogenase (G6P-DH)
ROCHE/10127655001 - grade I, from yeast
Synonym: G6P-DH; Glucose-6-Phosephate Dehydrogenase
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | yeast |
| color | white to yellowish |
| concentration | ≥0.1-1.0 % (w/w) |
| foreign activity | 6-PGDH <0.0100% |
| CK <0.00100% | |
| GR <0.0100% | |
| HK <0.0100% | |
| PGI <0.00200% | |
| PGluM <0.0100% | |
| form | suspension |
| manufacturer/tradename | Roche |
| NCBI accession no. | CAA93357   |
| optimum pH | 9.2 |
| packaging | pkg of 1 mL (5 mg/ml) |
| Quality Level | 100 |
| shipped in | wet ice |
| solubility | water: miscible |
| specific activity | ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.) |
| storage temp. | 2-8°C |
| suitability | suitable for UV spectrophotometry and general use |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P11412 |
| Application: | Component of cofactor recycling systems for NADPH. |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphat |
| General description: | Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP+ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP+ and NADPH levels the equilibrium shifts to inactive tetramers. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, pH approximately 6 |
| Preparation Note: | Activator: Mg2+ (5 to 10 mM) |
| Specifications: | Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI |
| Specifications: | Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |