NADH

ROCHE/10128023001 - Grade II, disodium salt

Synonym: β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate; β-DPNH; β-NADH; DPNH; Diphosphopyridine nucleotide, reduced form; NADH

CAS Number: 606-68-8 (anhydrous)
Empirical Formula (Hill Notation): C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O
Molecular Weight: 709.40 (anhydrous basis)
MDL Number: MFCD03791273
Linear Formula: C₂₁H₂₇N₇Na₂O₁₄P₂ · xH₂O
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-10128023001	1 g	$167.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ_max	260 and 340 nm
assay	98%
form	amorphous powder
InChI	1S/C21H29N7O14P2.2Na.H2O/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33;;;/h1,3-4,7-8,10-11,13-16,20-21,29-32H,2,5-6H2,(H2,23,33)(H,34,35)(H,36,37)(H2,22,24,25);;;1H2/q;2*+1;/p-2/t10-,11-,13-,14-,15-,16-,20-,21-;;;/m1.../s1
InChI key	FWBNCIFELNNJCX-UHOVGGJYSA-L
manufacturer/tradename	Roche
mol wt	(NADH-Na₂: M_r = 709.4 NADH: M_r= 665.4)
packaging	pkg of 1 g
Quality Level	100
shipped in	wet ice
SMILES string	O.[Na+].[Na+].NC(=O)C1=CN(C=CC1)[C@@H]2O[C@H](COP([O-])(=O)OP([O-])(=O)OC[C@H]3O[C@H]([C@H](O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
storage condition	protect from light

Analysis Note:	Purity: 98% β-NADH-Na₂ based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A₂₆₀ nm-A₃₄₀ nm: <2.40 Organic solvents: <4% (GC)
Application:	As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Application:	NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay.
Application:	NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay.
Biochem/physiol Actions:	NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD⁺/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations.
Features and Benefits:	Absorbance coefficients: 14.3 x 10³ l x mol^-1 x cm^-1 at 260 nm, 6.18 x 10³ l x mol^-1 x cm^-1 at 334 nm (Hg), 6.3 x 10³l x mol^-1 x cm^-1 at 340 nm, 3.4 x 10³ l x mol^-1 x cm^-1 at 365 nm (Hg) Formula: C₂₁H₂₇N₇O₁₄P₂Na₂ pH value in water: 7.5 ± 0.5 (100 mg/ml)
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Packaging:	Packaged by solid weight.
Physical form:	Amorphous powder
Preparation Note:	Activator: Mg²⁺ (5 to 10 mM) Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C
Preparation Note:	Store at 2 to 8 °C. (Store dry!)

RIDADR	NONH for all modes of transport
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	98%
UNSPSC	12352204

To Order From a Quote