NADH
ROCHE/10128023001 - Grade II, disodium salt
Synonym: β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate; β-DPNH; β-NADH; DPNH; Diphosphopyridine nucleotide, reduced form; NADH
CAS Number: 606-68-8 (anhydrous)
Empirical Formula (Hill Notation): C21H27N7Na2O14P2 · xH2O
Molecular Weight: 709.40 (anhydrous basis)
MDL Number: MFCD03791273
Linear Formula: C21H27N7Na2O14P2 · xH2O
Product Type: Chemical
| λmax | 260 and 340 nm |
| assay | 98% |
| form | amorphous powder |
| InChI | 1S/C21H29N7O14P2.2Na.H2O/ |
| InChI key | FWBNCIFELNNJCX-UHOVGGJYSA |
| manufacturer/tradename | Roche |
| mol wt | (NADH-Na2: Mr = 709.4 NADH: Mr = 665.4) |
| packaging | pkg of 1 g |
| Quality Level | 100  |
| shipped in | wet ice |
| SMILES string | O.[Na+].[Na+].NC(=O)C1=CN |
| storage condition | protect from light |
| Application: | As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
| Application: | NADH has been used in lactate dehydrogenase (LDH) enzymatic activity assay, glutamate-cysteine ligase activity assay and fructose 2,6-bisphosphate assay. |
| Application: | NADH has been used in MDH (malate dehydrogenase) enzymatic activity assay. |
| Biochem/physiol Actions: | NADH is a coenzyme that functions as a regenerating electron donor in catabolic processes including glycolysis, β-oxidation and the citric acid cycle (Krebs cycle, TCA cycle). It participates in cell signaling events as well, for example as a substrate for the poly (ADP-ribose) polymerases (PARPs) during the DNA damage response. The NAD+/NADH dependent sirtuins play key roles in stress responses during events involving energy metabolism, with implications in cancer biology, diabetes and neurodegenerative disease. As a reagent, NADH can be used in enzyme cycling assays to amplify detection of activity of biologically relevant enzymes or metabolites present in low concentrations. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | Packaged by solid weight. |
| Physical form: | Amorphous powder |
| Preparation Note: | Activator: Mg2+ (5 to 10 mM) Storage conditions (working solution): 10 mg/ml in Na-bicarbonate buffer, pH 9, contains at least 95% NADH after 4 weeks at 2 to 8 °C |
| Quality: | Purity: 98% β-NADH-Na2 based on dry weight (enzymatically and absorbance at 340 nm), 99% (absorbance at 260 nm), A260 nm-A340 nm: <2.40 Organic solvents: <4% (GC) |
| Specifications: | Absorbance coefficients: 14.3 x 103 l x mol-1 x cm-1 at 260 nm, 6.18 x 103 l x mol-1 x cm-1 at 334 nm (Hg), 6.3 x 103 l x mol-1 x cm-1 at 340 nm, 3.4 x 103 l x mol-1 x cm-1 at 365 nm (Hg) Formula: C21H27N7O14P2Na2 pH value in water: 7.5 ± 0.5 (100 mg/ml) |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 98% |
| UNSPSC | 12352204 |