Glucose-6-Phosphate Dehydrogenase (G6P-DH)
ROCHE/10165875001 - from Leuconostoc mesenteroides
Synonym: G6P-DH; Glucose-6-Phosephate Dehydrogenase
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | bacterial (Leuconostoc mesenteroides) |
| color | white |
| concentration | ≥0.1-1.0 % (w/w) |
| foreign activity | 6-PGDH <0.001% |
| CK <0.001% | |
| GR <0.01% | |
| HK <0.05% | |
| NADH oxidase <0.02% | |
| PGI <0.01% | |
| form | solution |
| suspension | |
| manufacturer/tradename | Roche |
| mol wt | dimer 110 kDa |
| NCBI accession no. | AAA25265   |
| optimum pH | 7.0-8.5(maximal activity at 7.8) |
| packaging | pkg of 1 mL (1,000 U) |
| Quality Level | 100 |
| shipped in | wet ice |
| solubility | water: miscible |
| specific activity | ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P11411 |
| Analysis Note: | Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm) |
| Analysis Note: | Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH |
| Application: | Component of cofactor recycling systems for NADPH. |
| Application: | It was used in enzymatic determination of C6 phosphorylation of glycogen. |
| Biochem/physiol Actions: | |
| General description: | Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1 |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8. |
| Physical form: | Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6 |
| Preparation Note: | Activator: HCO3- (≤ 0.3 M) activates slightly. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |