RNA, MS2
ROCHE/10165948001 - from bacteriophage MS2
Synonym: RNA; ribonucleic acid
Product Type: Chemical
biological source | bacteriophage MS2 |
color | colorless |
form | solution |
manufacturer/tradename | Roche |
mol wt | ~1200 kDa |
packaging | pkg of 500 μL (10 A260 units) |
Quality Level | 100 |
solubility | water: miscible |
storage temp. | −20°C (−15°C to −25°C) |
technique(s) | RNA extraction: suitable |
Application: | RNA, MS2 has been used: • as a standard to assess RNA quantification methods • in RNA extraction • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis. • for structural and functional studies. • to stabilize RNA during cDNA synthesis • to improve RNA yield during RNA extraction or isolation |
General description: | RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0. |
Quality: | Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures. |
Sequence: | Chain Length 3,569 nucleotides |
RIDADR | NONH for all modes of transport |
WGK Germany | nwg |
Flash Point(F) | No data available |
Flash Point(C) | No data available |
Storage Temp. | −20°C (−15°C to −25°C) |
UNSPSC | 41106311 |