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Glutamate Dehydrogenase (GlDH)

ROCHE/10197734001 - from beef liver

Synonym: DNA marker

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10197734001 3000 units
$117.00
1/EA
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application(s) life science and biopharma
biological source bovine liver
color white
concentration ≥10-20 % (w/w)
foreign activity ADH <0.00500%
  LDH <0.00500%
  MDH <0.00500%
form lyophilized
manufacturer/tradename Roche
optimum pH 8
packaging pkg of 3,000 U
Quality Level 100 
shipped in wet ice
solubility water: 20 mg/mL, colorless
specific activity 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.)
  ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
storage temp. 2-8°C
suitability suitable for UV spectrophotometry and general use
technique(s) activity assay: suitable
UniProt accession no. P00366 
Application: Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.
General description: GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Quality: Contaminants: <0.005% ADH, LDH, and MDH, each
Sequence: The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
activity specific activity: 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.); specific activity: ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 1.4.1.3   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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