Glutamate Dehydrogenase (GlDH)
ROCHE/10197734001 - from beef liver
Synonym: DNA marker
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | bovine liver |
| color | white |
| concentration | ≥10-20 % (w/w) |
| foreign activity | ADH <0.00500% |
| LDH <0.00500% | |
| MDH <0.00500% | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| optimum pH | 8 |
| packaging | pkg of 3,000 U |
| Quality Level | 100  |
| shipped in | wet ice |
| solubility | water: 20 mg/mL, colorless |
| specific activity | 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.) |
| ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.) | |
| storage temp. | 2-8°C |
| suitability | suitable for UV spectrophotometry and general use |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P00366  |
| Application: | Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H. |
| General description: | GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Quality: | Contaminants: <0.005% ADH, LDH, and MDH, each |
| Sequence: | The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.); specific activity: ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.4.1.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |