Glutamate Dehydrogenase (GlDH)
ROCHE/10197734001 - from beef liver
Synonym: DNA marker
Product Type: Chemical
application(s) | life science and biopharma |
biological source | bovine liver |
color | white |
concentration | ≥10-20 % (w/w) |
foreign activity | ADH <0.00500% |
LDH <0.00500% | |
MDH <0.00500% | |
form | lyophilized |
manufacturer/tradename | Roche |
optimum pH | 8 |
packaging | pkg of 3,000 U |
Quality Level | 100 ![]() |
shipped in | wet ice |
solubility | water: 20 mg/mL, colorless |
specific activity | 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.) |
~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.) | |
storage temp. | 2-8°C |
suitability | suitable for UV spectrophotometry and general use |
technique(s) | activity assay: suitable |
UniProt accession no. | P00366 ![]() ![]() |
Application: | Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H. |
General description: | GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Quality: | Contaminants: <0.005% ADH, LDH, and MDH, each |
Sequence: | The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD). |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
activity | specific activity: 10 U/mg (Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.); specific activity: ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.) |
Storage Temp. | 2-8°C |
Enzyme Commission (EC) Number | 1.4.1.3 ![]() ![]() ![]() ![]() ![]() |
UNSPSC | 12352204 |