GTP-γ-S
ROCHE/10220647001 - Tetralithium salt
Synonym: GTP-γ-S; GTP
Product Type: Chemical
| assay | 87% (HPLC) |
| color | white |
| composition | GDP, ≤12% HPLC |
| GMP, ≤1% HPLC | |
| GTP, ≤2% HPLC | |
| concentration | 97% |
| description | C10H12N5O13P3SLi4 |
| form | solid |
| manufacturer/tradename | Roche |
| mol wt | Mr 539.2 (GTP-γ-S) |
| Mr 563.0 (GTP-γ-S-Li4) | |
| packaging | pkg of 10 mg |
| Quality Level | 100  |
| solubility | water: soluble |
| storage condition | protect from light |
| storage temp. | −20°C (−15°C to −25°C) |
| technique(s) | activity assay: suitable |
| Analysis Note: | Absorption: Content is measured in aqueous solution by absorption at 254 nm. Conditions:• approx. 10 mg/ml double-dist. water • dilution: 9.8 ml double-dist. water + 0.2 ml sample. |
| Application: | GTP-γ-S has been used: • in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4) • in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα) • to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants |
| Biochem/physiol Actions: | Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-bindin |
| General description: | Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | 87% (HPLC) |
| Storage Temp. | −20°C (−15°C to −25°C) |
| UNSPSC | 12352204 |