Neuraminidase (Sialidase)
ROCHE/10269611001 - from Arthrobacter ureafaciens
Synonym: PCR; taq
Product Type: Chemical
Neuraminidase (Sialidase)
| biological source | bacterial (Arthrobacter ureafaciens) |
| form | solution |
| manufacturer/tradename | Roche |
| optimum pH | 5.0-5.5 |
| packaging | pkg of 1 U (100 μl) |
| Quality Level | 100  |
| specific activity | ~25 units/mg protein |
| storage temp. | 2-8°C |
| Application: | • cell surface lectin array analysis. • hemagglutination assays. • cell adhesion assay. |
| Application: | For the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials. |
| Application: | Neuraminidase has been used for the: • detection of the cell surface glycosylations in human anaplastic large cell lymphoma cells • release of sialic acid from cells • antibody-overlay lectin microarray |
| Biochem/physiol Actions: | Cleaves terminal sialic-acid residues that are α2,3-, α2,6-, or α2,8-linked to Gal, GlcNAc, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids, or glycoproteins. Relative rate of cleavage is α2,6 >α2,3 >α2,8, determined on bonds in tri- and tetrasaccharides. |
| General description: | Neuraminidase hydrolyzes terminal N- or O-acylneuraminic acids which are α2,3-, α2,6-, or α2,8-linked (rate: α2,6 > α2,3 > α2,8) to oligosaccharides, polysaccharides, mucopolysaccharides, glycoproteins, and glycolipids. Noteworthy, for the hydrolysis of glycolipids, the presence of a detergent is necessary. Because of the broad substrate specificity, the enzyme is very well suited for the complete removal of sialic acids from glycoconjugates of a wide variety of biological materials. |
| General description: | This product is a mixture of isoenzymes (L, M1, M2 and S) with the following molecular weight values: ~ 52 kDa, 66 kDa and 88 kDa. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution in 10 mM sodium phosphate, 0.1% Micr-O-Protect (w/v), 0.25 mg/ml bovine serum albumin, pH 7 |
| Preparation Note: | Working concentration: Enzyme/substrate ratio should be in the range of 0.04 U/25-80 μg. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~25 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.18 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |