Colcemid
ROCHE/10295892001 - solution, suitable for blocking, sterile; 0.2 μm filtered
Synonym: Demecolcine; N-
CAS Number: 477-30-5
Empirical Formula (Hill Notation): C21H25NO5
Molecular Weight: 371.43
MDL Number: MFCD00075459
Linear Formula: C21H25NO5
Product Type: Chemical
| description | N-methyl-N-deacetyl-colchicine |
| form | solution |
| impurities | microbial, tested |
| InChI | 1S/C21H25NO5/c1-22-15-8-6 |
| InChI key | NNJPGOLRFBJNIW-HNNXBMFYSA |
| manufacturer/tradename | Roche |
| packaging | pkg of 20 mL (10 μg/ml) |
| Quality Level | 100  |
| SMILES string | CN[C@H]1CCc2cc(OC)c(OC)c( |
| solubility | water: miscible |
| sterility | sterile; 0.2 μm filtered |
| storage temp. | 2-8°C |
| technique(s) | blocking: suitable |
| Application: | Colcemid inhibits the formation of mitotic spindles. It is used to increase the percentage of metaphase cells for chromosome analysis. |
| Biochem/physiol Actions: | Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase. |
| General description: | Colcemid is also known as demecolcine. Its generic name is N-methyl-N-deacetyl-colchicine. Colcemid depolymerizes microtubules and blocks mitosis at metaphase. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution (10 μg/ml), filtered through 0.2 μm pore size membrane. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |