Citrate Lyase (CL)
ROCHE/10354074001 - from Klebsiella pneumoniae (Aerobacter aerogenes)
Synonym: Citrate Lyase (CL)
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | bacterial (Klebsiella pneumoniae) |
| color | light cream |
| foreign activity | ICDH <0.05% (NAD as coenzyme) |
| NADH oxidase <0.05% | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| NCBI accession no. | WP_323133469   |
| optimum pH | 8.0-9.0 |
| packaging | pkg of 120 U |
| Quality Level | 100 |
| solubility | water: soluble |
| specific activity | ≥0.25 U/mg (At 25 °C with citrate as the substrate.) |
| storage temp. | 2-8°C |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P45413 |
| Application: | It was used in enzymatic assay to measure citrate concentration using microtiter plates. |
| General description: | Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate, stabilized with BSA, sucrose, MgSO4 and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized. |
| Preparation Note: | Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+) |
| Quality: | Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ≥0.25 U/mg (At 25 °C with citrate as the substrate.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.1.3.6 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |