β-Galactosidase
ROCHE/10745731001 - EIA grade
Synonym: β galactosidase; β-galactosidase
Product Type: Chemical
| λmax | 405 |
| application(s) | life science and biopharma |
| assay | (single peak, HPLC) |
| biological source | Escherichia coli |
| color | white |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 540.000 kDa |
| NCBI accession no. | WP_050940144   |
| packaging | pkg of 25 mg (approx. 100 mg lyophilizate) |
| pH | 7.3-7.7 |
| Quality Level | 100 |
| shipped in | dry ice |
| solubility | water: 20 mg/mL |
| specific activity | 750-950 U/mg |
| storage temp. | −20°C (−15°C to −25°C) |
| suitability | suitable for immunoassay |
| technique(s) | enzyme immunoassay: suitable |
| UniProt accession no. | P00722 P00722 |
| Analysis Note: | Specific activity: approximately 750 - 950 U/mg enzyme protein ≅ approximately 150 - 250 U/mg lyophilizate (+37°C, 2-nitrophenyl-β-D-galacto |
| Application: | β-Galactosidase is used for labeling enzyme immunoassay techniques. β-Galactosidase can be coupled to other proteins via its SH-groups. The reconstituted solution can be used directly for conjugation without prior dialysis or gel permeation chromatography. |
| Application: | β-Galactosidase has been used as a standard to determine the absolute quantitation of LacZ protein. |
| Biochem/physiol Actions: | β-Galactosidase catalyzes the hydrolysis of terminal β-galactose residue of ganglioside substrates, such as glycoproteins, sphingolipids, and keratan sulfate and other glycoconjugates. This enzyme is associated with the mechanism of cell senescence and carcinogenesis. Mutations in the gene result in gangliosidosis, an autosomal recessive disorder, characterized with defective lysosomal storage due to accumulation of substrates. β-Galactosidase deficiency also causes Morquio B syndrome indicating skeletal abnormalities, short stature and increased excretion of keratan sulfate in urine. |
| General description: | β-Galactosidase, EIA grade, is a lyophilizate from E. coli overproducer, consisting of enzyme protein, phosphate buffer, and sucrose. Substances which could interfere with the derivatization of NH2 or SH groups (e.g., 2-mercaptoethanol, ammonium salts, primary amines etc.) have been removed. |
| General description: | The GLB1 (β-Galactosidase) gene is mapped to human chromosome 3p22.3. The encoded protein belongs to glycosyl hydrolase 35 family and is localized to lysosomes. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate, stabilized with phosphate buffer and sucrose. Note: Contains at least 12 free SH-groups/enzyme molecule; 5 mg approximately 20 mg lyophilizate; 25 mg approximately 100 mg lyophilizate. |
| Quality: | Purity: single peak (HPLC) |
| Sequence: | Free Thiol Groups The non-denatured, enzymatically-active preparation contains > 12 SH groups per molecule which are not involved in disulfide bridges and are freely accessible to coupling reagents in aqueous media (as assayed with Ellman′s reagent at +37 °C, acc. to Habeeb, 1972). Absence of Enzyme Aggregates The preparation contains < 3% dimers (HPLC, area %) and essentially no higher oligomers. |
| Storage and Stability: | Store at -15–-25 °C. (sealed under nitrogen) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | (single peak, HPLC) |
| activity | specific activity: 750-950 U/mg |
| Storage Temp. | −20°C (−15°C to −25°C) |
| Enzyme Commission (EC) Number | 3.2.1.23 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |