DNA, lambda
ROCHE/10745782001 - from bacteriophage lambda cl857 Sam 7
Synonym: lambda dna
Product Type: Chemical
| color | colorless |
| concentration | 250 μg/mL |
| form | solution |
| impurities | nuclease, none detected |
| RNA, none detected | |
| manufacturer/tradename | Roche |
| mol wt | 32,300 kDa |
| packaging | pkg of 1 mL |
| Quality Level | 100  |
| shipped in | dry ice |
| solubility | water: miscible |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C (−15°C to −25°C) |
| Application: | DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate. |
| General description: | The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5’ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use |
| Quality: | The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III. |
| Sequence: | Chain Length 48,502 bp |
| Storage and Stability: | Store at -15 to -25°C until use; once thawed, store at 2-8 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C (−15°C to −25°C) |
| UNSPSC | 41106310 |