Ribonuclease H (RNase H)
ROCHE/10786357001 - from Escherichia coli H 560 pol A1
Synonym: rnase h
Product Type: Chemical
application(s) | life science and biopharma |
assay | 100% |
biological source | Escherichia coli ( |
color | colorless |
foreign activity | endonuclease ~10 units, none detected (using lambda-DNA) |
nicking activity 10 units, none detected | |
RNase, none detected (up to 10 U with MS- II- RNA) | |
form | solution |
manufacturer/tradename | Roche |
NCBI accession no. | NP_414750.1 ![]() ![]() |
optimum pH | 7.5-9.1 |
packaging | pkg of 100 U |
Quality Level | 100 ![]() |
shipped in | dry ice |
solubility | water: miscible |
specific activity | ~40000 units/mg protein |
storage temp. | −20°C (−15°C to −25°C) |
suitability | suitable for molecular biology |
technique(s) | cDNA synthesis: suitable |
Application: | Ribonuclease H (RNase H) has been used for: • In vivo RNA-primed initiation of DNA synthesis • Elimination of mRNA during second-strand cDNA synthesis • Site-specific cleavage of RNA • Detection of RNA:DNA regions in double-stranded DNA of natural origin • Removal of poly (A) sequences of mRNA if oligo (dT) is present • RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) |
Biochem/physiol Actions: | Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication. |
Features and Benefits: | • Eliminate potential sources of PCR errors. • Increase accessibility of primers during subsequent PCR. |
General description: | Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. Source: E. coli H560 pol A1 Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C) Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer. |
General description: | Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. Using RNase H after the cDNA synthesis step can increase the sensitivity of a two-step RT-PCR assay. |
Preparation Note: | Activator: The enzyme has its maximal activity in presence of SH-reagents |
Quality: | Absence of endonucleases, nicking activities, and ribonucleases. |
Storage and Stability: | Store at -15–-25 °C. (unopened) |
Unit Definition: | RNase H is assayed according to Hillenbrand and Staudenbauer. One unit of RNase H is the amount of enzyme which produces 1 nmol acid-soluble ribonucleotides from[3H] poly(A) x poly(dT) in 20 minutes at +37 °C under the stated assay conditions. Volume Activity: Approximately 1 U/μl |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | 100% |
activity | specific activity: ~40000 units/mg protein |
Storage Temp. | −20°C (−15°C to −25°C) |
UNSPSC | 12352204 |