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Ribonuclease H (RNase H)

ROCHE/10786357001 - from Escherichia coli H 560 pol A1

Synonym: rnase h

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10786357001 100 units
$520.00
1/EA
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application(s) life science and biopharma
assay 100%
biological source Escherichia coli ( H 560 pol A1)
color colorless
foreign activity endonuclease ~10 units, none detected (using lambda-DNA)
  nicking activity 10 units, none detected
  RNase, none detected (up to 10 U with MS- II- RNA)
form solution
manufacturer/tradename Roche
NCBI accession no. NP_414750.1 
optimum pH 7.5-9.1
packaging pkg of 100 U
Quality Level 100 
shipped in dry ice
solubility water: miscible
specific activity ~40000 units/mg protein
storage temp. −20°C (−15°C to −25°C)
suitability suitable for molecular biology
technique(s) cDNA synthesis: suitable
Application: Ribonuclease H (RNase H) has been used for:
• In vivo RNA-primed initiation of DNA synthesis
• Elimination of mRNA during second-strand cDNA synthesis
• Site-specific cleavage of RNA
• Detection of RNA:DNA regions in double-stranded DNA of natural origin
• Removal of poly (A) sequences of mRNA if oligo (dT) is present
• RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)
Biochem/physiol Actions: Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Using RNase H for degrading mRNA results in 80% depletion of mRNA and protein expression. RNase H recognizes the start codon and the 3′ and 5′ untranslated regions. This enzyme participates in DNA replication.
Features and Benefits: • Eliminate potential sources of PCR errors.
• Increase accessibility of primers during subsequent PCR.
General description: Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.
General description: Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.
Other Notes: For life science research only. Not for use in diagnostic procedures. Using RNase H after the cDNA synthesis step can increase the sensitivity of a two-step RT-PCR assay.
Preparation Note: Activator: The enzyme has its maximal activity in presence of SH-reagents
Quality: Absence of endonucleases, nicking activities, and ribonucleases.
Storage and Stability: Store at -15–-25 °C. (unopened)
Unit Definition: RNase H is assayed according to Hillenbrand and Staudenbauer. One unit of RNase H is the amount of enzyme which produces 1 nmol acid-soluble ribonucleotides from[3H] poly(A) x poly(dT) in 20 minutes at +37 °C under the stated assay conditions.

Volume Activity: Approximately 1 U/μl
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) does not flash
Flash Point(C) does not flash
Purity 100%
activity specific activity: ~40000 units/mg protein
Storage Temp. −20°C (−15°C to −25°C)
UNSPSC 12352204

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