Endoproteinase Glu-C (V8 Protease)
ROCHE/10791156001 - from Staphylococcus aureus V8
Synonym: V8 protease; protease v8
Product Type: Chemical
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| mol wt | 30 kDa |
| optimum pH | 8.0-8.5 |
| packaging | pkg of 2 mg |
| specific activity | 20 U/mg |
| Application: | Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis. |
| Biochem/physiol Actions: | Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes. |
| Biochem/physiol Actions: | Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used. |
| General description: | Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranili |
| General description: | At 25 °C with Z-Phe-Leu-Glu-4-nitranili |
| General description: | Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: The enzyme has its maximal activity in presence of SH-reagents Working concentration: 1 to 5 mM Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml). Storage conditions (working solution): -15 to -25 °C The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once. |
| Preparation Note: | Store at 2 to 8 °C. (Store dry!) |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: 20 U/mg |
| Enzyme Commission (EC) Number | 3.4.21.19  ( BRENDA  | IUBMB ) |
| UNSPSC | 23201100 |