Endoproteinase Glu-C (V8 Protease)

ROCHE/10791156001 - from Staphylococcus aureus V8

Synonym: V8 protease; protease v8

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-10791156001	2 mg	$203.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	lyophilized (salt-free)
manufacturer/tradename	Roche
mol wt	30 kDa
optimum pH	8.0-8.5
packaging	pkg of 2 mg
specific activity	20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).)

Application:	Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.
Biochem/physiol Actions:	Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
General description:	At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
General description:	Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Preparation Note:	Activator: The enzyme has its maximal activity in presence of SH-reagents Working concentration: 1 to 5 mM Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml). Storage conditions (working solution): -15 to -25 °C The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Specificity:	Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.
Storage and Stability:	Store at 2 to 8 °C. (Store dry!)

Symbol	GHS07,GHS08
Signal word	Danger
Hazard statements	H315 - H319 - H334 - H335
Precautionary statements	P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	does not flash
Flash Point(C)	does not flash

activity	specific activity: 20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).)
Enzyme Commission (EC) Number	3.4.21.19 ( BRENDA \| IUBMB )
UNSPSC	23201100

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