BM-Cyclin
ROCHE/10799050001 - lyophilized, active toward mycoplasma
Synonym: antibiotic; minocycline; tiamulin
Product Type: Chemical
| antibiotic activity spectrum | mycoplasma |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mode of action | protein synthesis | interferes |
| packaging | pkg of 37.5 mg (for 2 x 2.5 l medium) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects. |
| General description: | BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | Set of two different antibiotics |
| Physical form: | Vial 1 (Tiamulin-hydrogenfumarat Vial 2 (Minocyclinhydrochloride) |
| Preparation Note: | Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2). Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines. For hybridomas decrease concentration of BM-Cyclin by 50%. Preparation of DAPI Working Solution Dilute the stock solution with methanol to a final concentration of 1μg/ml. Working solution: Preparation of Stock Solution Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water. Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution Dissolve DAPI in water to a final concentration of 1 to 5mg/ml. Note: Do not use any buffers. This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots. Storage conditions (working solution): -15 to -25°C Reconstituted stable at -15 to -25°C for at least 6 months. |
| Specificity: | Sensitive Organisms: Mycoplasmas and bacteria • BM Cyclin was found to effectively eliminate Acholesplasma laidlawii, • Mycoplasma arginini, • Mycoplasma hyorhinis, and • Mycoplasma orale from experimentally contaminated and chronically infected cell lines. Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H315 - H319 - H335 |
| Precautionary statements | P261 - P264 - P280 - P304 + P340 + P312 - P337 + P313 - P403 + P233 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |
| Components | BM-Cyclin 1: pleuromutilin derivative; BM-Cyclin 2: tetracycline derivative |