Tris hydrochloride
ROCHE/10812846001 - >99% (titration), pH 7.0-9.0, suitable for FISH
Synonym: Trizma® hydrochloride; TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)
CAS Number: 1185-53-1
Empirical Formula (Hill Notation): C4H11NO3 · HCl
Molecular Weight: 157.60
MDL Number: MFCD00012590
Linear Formula: NH2C(CH2OH)3 · HCl
Product Type: Chemical
| absorption | <0.02 at 300 nm at 100 mg/mL |
| assay | >99% (titration) |
| form | solid |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6, |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA |
| manufacturer/tradename | Roche |
| packaging | pkg of 500 g |
| pH | 7.0-9.0 |
| pKa (25 °C) | 8.1 |
| SMILES string | Cl.NC(CO)(CO)CO |
| storage temp. | room temp |
| technique(s) | FISH: suitable |
| useful pH range | 7.0-9.0 |
| Analysis Note: | Purity: >99% Tris-HCl (titrimetric) Heavy metals: <1 ppm Pb and Fe |
| Application: | Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. |
| Features and Benefits: | Crystals |
| Legal Information: | Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research   |
| Other Notes: | The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >99% (titration) |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |