Primer for cDNA Synthesis
ROCHE/10814270001 - lyophilized, pkg of 40 μg
Synonym: oligo-p(dt)15
Product Type: Chemical
form | lyophilized |
manufacturer/tradename | Roche |
packaging | pkg of 40 μg |
Quality Level | 100 |
storage temp. | −20°C (−15°C to −25°C) |
Application: | Primer for cDNA synthesis may be used in: • RT-PCR • Generation of cDNA libraries • Synthesis of first-strand cDNA |
General description: | This primer consists of a 15-mer oligonucleotide that shows thymidine (T) at each individual position. The oligonucleotides are HPLC purified, desalted, and supplied as a lyophilizate of the Li-salt. The p(dT)15 primer is not phosphorylated at the 5′ end.Oligo(dT)N generates full-length cDNAs. If template contains oligo(A) stretches internally, the primer may bind these, causing mis priming and transcripts that are not full-length. Composition: p(dT)15[ 15-mer oligonucleotide containing only deoxythymidine (dT) residues]. Phosphorylation: The p(dT)15 primer is not phosphorylated at the 5′ end. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Unit Definition: | 8 nmol ξ 1 A260 unit |
RIDADR | NONH for all modes of transport |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | −20°C (−15°C to −25°C) |
UNSPSC | 41106300 |