Peroxidase (POD)
ROCHE/10814407001 - EIA grade, from horseradish
Synonym: POD
Product Type: Chemical
| application(s) | detection life science and biopharma |
| assay | 90% (HPLC) |
| biological source | horseradish |
| color | red to brown |
| concentration | 10 g/L |
| foreign activity | AcP <0.00100% |
| ATPase <0.00100%, none detected | |
| Catalase <0.700% | |
| form | lyophilized (salt-free) |
| manufacturer/tradename | Roche |
| optimum pH | 6.0-6.5 |
| packaging | pkg of 100 mg |
| Quality Level | 100  |
| shipped in | dry ice |
| solubility | water: soluble at 68 °F |
| specific activity | ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2) |
| storage temp. | −20°C (−15°C to −25°C) |
| suitability | suitable for molecular biology |
| technique(s) | activity assay: suitable |
| UniProt accession no. | P00433  |
| Application: | Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay. |
| General description: | Donor: hydrogen-peroxide oxidoreductase Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG). Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H2O2) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H2O2, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H2O2) Amino groups: 2 - 3moles/mole enzyme |
| Preparation Note: | Carbohydrates: 12.0 - 14.5% (w/w) Isoenzymes: ≥90% isoenzyme C (HPLC) Purity: single peak (HPLC) Purity number: 3.0 - 3.5 (A403/A275) |
| Sequence: | Blocking substances Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH2-groups/mol does not change. POD POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca2+. POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes). Carbohydrate Content 12 to 14.5 mg/100 mg Iyophilizate Free Amino Groups Free amino groups: 2 to 3 mol/mol enzyme |
| Storage and Stability: | Keep container tightly closed in a dry and well-ventilated place. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 90% (HPLC) |
| activity | specific activity: ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2) |
| Storage Temp. | −20°C (−15°C to −25°C) |
| Enzyme Commission (EC) Number | 1.11.1.7 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |