DNA Molecular Weight Marker V
ROCHE/10821705001 - pkg of 50 μg (in 200 μl), solution
Synonym: DNA Marker
Product Type: Chemical
DNA Molecular Weight Marker V (4% agarose gel)
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (in 200 μl) |
| Quality Level | 100  |
| storage condition | avoid repeated freeze/thaw cycles |
| Application: | DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: <UL><LI>Restriction digests</LI> <LI>PCR</LI> <LI>RT-PCR</LI></UL> |
| Components: | Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0) |
| General description: | Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III. Size Range: 8 to 587 bp |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG). Storage conditions (working solution): 2 to 8 °C Once thawed we recommend further storage at 2 to 8 °C. |
| Sequence: | The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. |
| Storage and Stability: | Store at -15–-25 °C. (until use) |
| Unit Definition: | 50 μg = 1A260 unit |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 41105335 |