Lysozyme
ROCHE/10837059001 - from hen egg white
Synonym: Mucopeptide N-
Product Type: Chemical
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| absorption | 3.9 at 280 nm (10 mg enzyme/ml) |
| assay | ≥95% |
| biological source | chicken egg white |
| form | solid |
| manufacturer/tradename | Roche |
| mol wt | 14.4 kDa |
| optimum pH | 6.0-7.0 |
| packaging | pkg of 10 g |
| Quality Level | 100  |
| specific activity | >23000 U/mg (Shugar units) |
| Application: | Lysozyme has been used for: • Cell wall degradation • Preparation of protoplasts • Bacteriolysis • Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics) • Food and drinks (flavor enhancer) • Sample preparation prior to isolation of nucleic acids |
| Application: | Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation. |
| Biochem/physiol Actions: | Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria. |
| General description: | Muramidase, Mucopeptide N-acetylmuramoyl-hydrolas |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents. Storage conditions (working solution): -15 to -25 °C Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks. |
| Reconstitution: | Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use. |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| Unit Definition: | One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥95% |
| activity | specific activity: >23000 U/mg (Shugar units) |
| Enzyme Commission (EC) Number | 3.2.1.17 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |