Plasminogen
ROCHE/10874477001 - from human serum
Synonym: plasminogen
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | human serum |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | 90-94 kDa |
| packaging | pkg of 20 U |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.) |
| technique(s) | activity assay: suitable |
| Application: | Plasminogen is used for the determination of the activity of plasminogen activators. The enzyme is a substrate for tissue plasminogen activator (t-PA) in the t-PA test and a reference substance for t-PA therapy. It is used as the in-process control in the production of t-PA. |
| Features and Benefits: | Contents Lyophilizate, stabilized with bovine serum albumin. |
| General description: | Plasminogen is a profibrinolysin from human serum. Plasminogen, the proenzyme of the fibrinolytically active enzyme plasmin, is a single chain glycoprotein. Various isoelectric forms exist and can be separated by means of isoelectric focusing (IEF). The lyophilizate is stabilized with bovine serum albumin (BSA). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: stabilized with BSA Working solution: Solvent is recommended in 20 mM sodium phosphate buffer, pH 7.5, 100 mM NaCl. Storage conditions (working solution): -15 to -25 °C A solution in 20 mM sodium phosphate buffer, pH ca. 7.5, 100 mM NaCl is stable for several months without loss of activity when stored at -15 to -25 °C. Note: Plasminogen is susceptible to autolysis, which may result in activation. Contact with the skin or other contamination (e.g., by microorganisms), is therefore to be avoided. |
| Quality: | Contaminants: <0.05% plasmin; the preparation does not contain 6-aminohexanoic acid |
| Sequence: | Native plasminogen (glu-plasminogen) has a N-terminal glutamic acid group. Partial plasma proteolysis gives rise to a molecule with N-terminal lysine (lys-plasminogen). Cleavage with elastase results in val-plasminogen. The complete amino acid sequence contains 790 amino acids. Roche Applied Science supplies glu-plasminogen. |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~11 units/mg protein (activated with streptokinase at 37 °C and pH 7.4 with Chromozym PL as the substrate.) |
| UNSPSC | 12352204 |