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N-Acetyl-β-D-Glucosaminidase (NAG)

ROCHE/10875406001 - sufficient for ~50 tests

Synonym: NAG

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-10875406001 50 tests
 $734.00
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N-Acetyl-Β-D-Glucosaminidase (NAG)

 

form solid
manufacturer/tradename Roche
Quality Level 100 
storage temp. room temp
usage sufficient for ~50 tests
Application: N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
General description: N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: 1 kit containing 3 components
Preparation Note: Working solution: Preparation of solutions:
  1. Buffer solution
    Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
    • Substrate solution
    Dissolve the contents of bottle 2 with 55 ml solution I.
    • Stop reagent
    Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
Storage Temp. room temp
UNSPSC 12352200
Components Buffer Substance consisting of citric acid and potassium citrate; Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax; Stop Reagent consisting of sodium carbonate

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