Advanced Search



N-Acetyl-β-D-Glucosaminidase (NAG)

ROCHE/10875406001 - sufficient for ~50 tests

Synonym: NAG

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-10875406001 50 tests
$734.00
1/EA
Add To Favorites
N-Acetyl-Β-D-Glucosaminidase (NAG)

 

application(s) life science and biopharma
color white to light yellow-brown
form solid
manufacturer/tradename Roche
mol wt 105.99 g/mol
Quality Level 100 
solubility soluble ~71 g/L at 32 °F (stop reagent)
  water: soluble (Buffer substance, lyophilized substrate)
  water: soluble ~210 g/L at 68 °F (Stop reagent)
storage temp. room temp
suitability suitable for enzyme test
technique(s) activity assay: suitable
usage sufficient for ~50 tests
Application: N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.
General description: N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Packaging: 1 kit containing 3 components
Preparation Note: Working solution: Preparation of solutions:
  1. Buffer solution
    Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
    • Substrate solution
    Dissolve the contents of bottle 2 with 55 ml solution I.
    • Stop reagent
    Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.
Storage and Stability: Keep container tightly closed in a dry and well-ventilated place.
Storage Temp. room temp
UNSPSC 12352204
Components Buffer Substance consisting of citric acid and potassium citrate; Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax; Stop Reagent consisting of sodium carbonate

The following items have been added to your cart:

Choose a favorite list for this item:

Catalog Number Description Price
$

Returns/Order support

Please fill out the form below if you want to request order support from Krackeler Scientific.


Quick Order

* Required


New Year Price Updates

We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If you'd like the most current information sooner, please don't hesitate to drop us an email or give us a call and we'd be happy to assist. Thank you for your patience while we are updating.

800-334-7725
office@krackeler.com


Play Video

To Request a Quote

  1. Search or Browse for items and add to them to your Shopping Cart.
  2. Click the "Request Quote" button at the bottom of the Shopping Cart page.
  3. Fill out required fields.
  4. Optionally you can convert to standard checkout mode by choosing a payment type.
  5. Click "Request Quote" at the bottom of the page.

You will be contacted with a quote.

To Order From a Quote

  1. Register and login to the website.
  2. Receive a quote from your sales representative or customer service.
  3. Have your copy of the quote in hand.
  4. Visit our quote module to search for your quote.
Back to Top