Glycogen
ROCHE/10901393001 - from mussels
Synonym: Glycogen; glycogen
Product Type: Chemical
Glycogen
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mL (20 mg) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs. 20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml. In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured. |
| Features and Benefits: | Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations. Contents Aqueous solution, 20 mg/ml |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Quality: | Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |